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List of publications based on Milestone instrumentations/reagents.


Vacuum Preservation of Biospecimens - TissueSAFE/SealSAFE

Maria Comanescu, et al (2012),
“Critical Steps in Tissue Processing in Histopathology”,
Recent Patents on DNA & Gene Sequencing 2012, 6, 22-32.

Manfred Dietel, et al. (2015),
“Diagnostic procedures for non-small-cell lung cancer: recommendations of the European Expert Group”,
Thorax on line BMJ group, 2015.

Ian A.Cree et al fro the European Society of Pathology (2014),
“Guidance for laboratories performing molecular pathology for cancer patients”,
JCP on line BMJ group, July 2014.

Richard J.Zarbo et al. (2013),
“Validation of Tissue Preservation Using Vacuum Sealed Formalin-Free trasportation System”,
Poster, USCAP 2013.

Abbey P.Theiss. et al. (2014),
“Immunohistochemistry of Colorectal cancer Biomarker Phosphorylation requie Controlled Tissue Fixation”,
PLOS one, 2014.

Dhananjay A. Chitale, et al. (2015),
“Molecular Validation Study of Nucleic Acids Extraction from Vacuum Sealed Surgical Pathology Specimens”,
USCAP poster 2015.

Alberto Martinez Carrasco, et al. (2015),
“Del Quiròfano al biobanco: circuito de recogida de maestra de cancer de mama”,
Poster, 2015.

Agustin Perra Montoya, et al. (2015),
“Papel de biobanc-MUR, biobanco en red de la region de Murcia, en la creacion de la cohorte nutrition in early life and asthma: circuito de recogida de muestras”,
Poster, 2015.

Sergio Abenza Camacho, et al. (2015),
“Preservacion del ARN de Placenta conservada mediante sistema de alto vacio TissueSAFE: estudio piloto”,
Poster, 2015.

Villena C., et al. (2015),
“Ultimos avances en la evaluacio de la calidad de los tepido archi viados en los biobancos”,
Poster, 2015.

Martin I, et al. (2015),
“Incorporation de Tejidos en biobanco a traves de TissueSAFE”,
Poster, 2015.

Robies-Frias MJ, et al. (2015),
“Implantation de un circuito de preservacion de muestras de trasplante hepatico envasadas al vacio, en el Nodo Biobanco H.Virgen del Rocio-IBiS”,
Poster, 2015.

Manfred Dietel, et al (2015),
“Pre-analytics of Pathology Specimens in oncology”,
Book publised by Springer, 2015.

Karen Lester (2015),
“Reducing Formalin Exposure risks in Surgical Specimen Management”,
Poster, 2015.

Anna Sapino (2016),
“Reliable histopathological diagnoses”,
International Innovation, 2015.

Richard J.Zarbo et al. (2016),
“Validation of Placenta preservation for Pathologic examination using the TissueSAFE formalin-free vacuum sealing system”,
Poster, USCAP 2016.

Mark Terry (2014),
“Advances in Pathology tissue management reduce formalin use, improve quality and cut costs”,
White Paper, DARK daily Laboratory and Pathology News, 2014.

G. Bussolati, L. Chiusa, A. Cimino and G. D’Armento (2009),
“Under-Vacuum Preservation of Tissue to be Transferred to Pathology Labs”,
Department of Biomedical Sciences and Oncology, University of Turin, Turin, Italy.

G. Bussolati, L. Chiusa, A. Cimino, G. D’Armento (2007),
“Tissue transfer to pathology labs: under vacuum is the safe alternative to formalin”,
Springer-Verlag, 452:229–231

A. Sapino, G. D’Armento, L. Molinaro, L. Macrì, A. Cavicchini, G. Bussolati (2009),
“Gene Expression Profiling in Under-Vacuum Stored Breast Carcinomas: a Pilot Study”,
Department of Biomedical Sciences and Human Oncology - University of Turin - Italy

C. Di Novi, D. Minniti, S. Barbaro, M. G. Zampirolo, A. Cimino, G. Bussolati (2010),
“Vacuum-based preservation of surgical specimens: An environmentally-safe step towards a formalin-free hospital”,
Sci Total Environ, doi 10.1016/jscitotenv.2010.04.022

A.Moggio, G. D'armento and B. Bussolati (2012),
“Efficient stel cell isolation from under vacuum preserved tissue samples”,
Organogenesis 8:3, 1-5 July/August/September 2012

G. Bussolati, L. Annaratone and A. Sapino (2012),
“Vacuum sealing and cooling for safe transfer of tissues from operating theatre to laboratory”,
Poster, USCAP 2012

G.Bussolati (2011),
“RNA preservation in FFPE tissues requires pre-analytical standardized processing”,
Poster, USCAP 2011

J. F. Flejou, S. El-Naderi, P. Cervera (2013),
“Vacuum based preservation of colorectal cancer specimens: A comparison with formalin fixation”,
Medical school Pierre et Marie curie, Paris

David Butler et al. (2011),
“Keeping your Tissue-Safe (Equipment Trial)”,
Prince Charles Hospital Brisbane Queensland Government Australia RCPA Sydney Australia 2011

DP Boyle, R Carson, P Kelly, M Catherwood, S Carroll, L Venkatraman, S McQuaid, H McBride, J James, MB Loughrey (2010),
“Evaluation of tissue preservation using a vacuum-based refrigeration system for specimen transfer from theatre to laboratori”,
Department of Pathology, Royal Group of Hospitals Trust, Belfast, Northern Ireland

R.Martinez Marin et al. (2010),
“Traslado de muestras quirurgicas desde quirofano al Servici de Anatomia Patologica. Intraoperatoria “versus” Sistema de alto nivel vacio”,
BIOBANC – Mur HUVA FFIS IMIB ESBB Congress Tarragona 2010

Michael Dib PA (ASCP), Ruan C Varney CT, SSBB, Bruce Jones M.D. (2012),
Richard J. Zarbo M.D., “Histologic Validation of Vacuum Sealed Formali-Free Tissue Preservation and Transport System”,
Pathology & Laboratory Medicine Henry Ford Health System, Detroit, Michigan AAPA Congress USA 2012

I Marron V.Alvarez et al. (2012),
“Vacuum Sealing for a safe transfer of tissue from operating theatre to laboratory using TissueSafe Milestone apparaturatus”,
Hospital Universitario Central De Asturias - Spain ESBB Congress Granada 2012

M.Ferronato, B. Filippini, C.Rossetti, A.Scarpa (2012),
“Vacuum packed transport maintains the RNA quality”,
ABC-NET Applied Research on Cancer Verona- Italy ESBB Congress Granada 2012

M. Caly, A. Léon a, M. Galut b, O. Mariani b, E. Padoy a, X. Sastre-Garau (2013),
“Analyse qualitative et quantitative d’échantillons tissulaires par le procédé TissueSafe® en vue d’analyses morphologiques et moléculaires. Évaluation sur une série de 10 cas”,
CRB Centre de Ressources Biologiques Institut Curie. Paris – France Annales de Pathologie Vol 32 - N° 5S

B. Poulet, O. Languille, H. Lacluque, G. Hubervic, C. Adem, S. Azoulay, A. Barres (2013),
“Contrôle de la fixation des mastectomies par la procédure Tissue Safe®”,
Instutute Pathologie France Annales de Pathologie Vol 32 - N° 5S

M. Manzotti, A. Cavallon, M.O. Biasi, T.Tamagni, C. Camia, M. Schiavi, E.Pisa, G. Renne (2011),
“Evaluation of tissue preservation of material stored at 4°C with TissueSAFE system after the examination and macroscopic sampling”,
Poster, IEO European Institute of Oncology, Milan

P.Ntiamoah, et al (2016),
“EDTA-based decalcification of bone and bone marrow – ideal tool for protein and nucleic acid preservation – a pilot study”,
Memorial Sloan Kettering, USCAP poster, 2016

Ilse de Laak, et al. (2015),
“Rapid processing of bone marrow biopsies with excellent morphology, immunohistochemistry and DNA quality”,
Radboud University centre, Congress poster, 2015

Macro Digital Imaging

F. Joel W-M Leong, A. S-Y Leong (2004),
“Digital photography in anatomical pathology”,
J Postgrad Med, 50-1

A. S-Y Leong, F. Visinoni, C. Visinoni and J. Milios (2000),
“An Advanced Digital Image-Capture Computer System for Gross Specimens: a Substitute for Gross Description”,
Royal College of Pathologists of Australasia, 32. Pp. 131-135

Bill Ahlfeld (2012),
“Tips and Tricks”,
The Cutting Edge, The journal of AAPA, vol 2, 2012

D.A. Chitale, et al. (2016),
“Image documented surgical pathology (ID-SP) to enhance patient safety – Lean re design of the value stream steps form Gross examination to signout”,
USCAP poster, 2016

A.Hanif (2003),
“Image is everything as MacroPATH arrived in the cut-up room”,
Pathology in practice, vol 4, issue 3, 2003


P.Ntiamoah, et al,
“EDTA-based decalcification of bone and bone marrow – ideal tool for protein and nucleic acid preservation – a pilot study”,
Memorial Sloan Kettering, USCAP poster, 2016

Ilse de Laak, et al.,
“Rapid processing of bone marrow biopsies with excellent morphology, immunohistochemistry and DNA quality”,
Radboud University centre, Congress poster, 2015

A. Leong (2009),
“Effects of Microwave Fixation and Decalcification in Rodent Tissue”,
Pfizer Global Research & Development, La Jolla, CA.

Non Formalin Fixation - FineFIX

G. Stanta, S. Pozzi Mucelli, F. Petrera, S. Bonin and G. Bussolati (2006),
“A Novel Fixative Improves Opportunities of Nucleic Acids and Proteomic Analysis in Human Archive’s Tissue”,
Diagn Mol Pathol, 15-2.

A. Gazziero, R. Salmaso, V. GUzzardo, M. Zoletto, R. Salmaso, A. Fassina (2008),
“Alchoolic Fixative and Quality of Nucleic Acids”,
Department of Diagnostic Sciences and Special Therapy, University of Padua, Italy.

A. Gazziero, V. Guzzardo, E. Aldighieri and A. Fassina (2008),
“Morphological quality and nucleic acid preservation in cytopathology”,
Journal of Clinical Pathology.

B. Espina, R. Chuaqui, J. Rodriguez, S. Hewitt, C. Ghimenton, F. Visinoni, M. Bellini, C. Compton, J. Vaught; H. Moore, J. Schneider, J. Gillespie (2006),
“Determination of FineFix fixation on histologic quality ad recovery of RNA from human prostate tissue”.

GM Reynolds, BA O’Sullivan, J Junying, PP Taniere, NS Deshmukh (2005),
“A formalin-free fixative, that provides superior preservation for molecular techniques”,
Poster Presentation at IBMS meeting – Birmingham, UK

Antonio Iesurum, et al (2006),
“Microwave processing and Ethanol based fixation in Forensic Pathology”,
Am J Forensic Pathology, 27; 178-182, 2006

D.H. Pauza, et al.,
“Two years experience of formalin-free preservation of the human organs and total corpses for Anatomical Dissections in Kaunas University of Medicine”,
Institute of Anatomy, Anivesity of Medicine, Kaunas, Lithuania

Cathy B. Moleans, et al. (2011),
“Formaldehyde substitute fixatives”,
Am J Clin Pathol, 136; 548 – 556, 2011

Tissue Processing

F. Visinoni, J. Milios, A. S.-Y. Leong, M. E. Boon, L. P. Kok, F. Malcangi (1998),
“Ultra-Rapid Microwave/Variable Pressure-Induced Histoprocessing: Description of a New Tissue Processor”,
The Journal of Histotechnology / Vol. 21, No. 3

Karen Titus (2008),
“Vision quest – fresh look at AP automation”,
Cap Today, Vol. 22 No. 8

A. S.-Y. Leong and F. J. W-M Leong (2005),
“Strategies for laboratory cost containment and for pathologist shortage: centralised pathology laboratories with microwave-stimulated histoprocessing and telepathology”,
Pathology, pp. 1-5

A. S.-Y. Leong and David Price (2004),
“Incorporation of microwave tissue processing into a routine pathology laboratory: impact on turnaround times and laboratory work patterns”,
Pathology, 36(4), pp. 1-4

E. Rossomacha, C. D. Hoemann, A. Chevrier, M. S. Shive (2007),
“Articular Cartilage and Synovial Tissue”,
BioSYntech Inc.

L. Nava, M. Freschi, C.Doglioni, E. Scapaticci, A. Losa, M. Riva, P. Rigatti, and G. Guazzoni (2007),
“Definitive histologic diagnosis on prostate biopsies in 3 hours”,
22st Congress Of The European Association Of Urology, Abstr. 406.

K. Alderton, W. Dellow, M. Whitehead, G. Devane (2008),
“A Microwave Tissue Processor Trial in a Routine Hospital Analtomical Pathology Laboratory,
22st Congress Of The European Association Of Urology.

Antonio Iesurum, Tiziana Balbi, Domenico Vasapollo, Alberto Cicognani and Claudio Ghimenton (2006),
“Microwave Processing and Ethanol-Based Fixation in Forensic Pathology”,
The American Journal of Forensic Medicine and Pathology, 27-2.

T. Ragazzini, E. Magrini, M. C. Cucchi, M. Foschini and V. Eusebi (2005),
“The Fast-Track Biopsy (FTB): Description of Rapid Histology and Immunohistochemistry Methods for Evaluation of Preoperative Breast Core Biopsies”,
International Journal of Surgical Pathology, 13(3):247-252.

Zenobia A. M. Hafajee and Anthony S.-Y. Leong (2004),
“Ultra-rapid microwave-stimulated tissue processing with a modified protocol incorporating microwave fixation”,
Pathology, 36(4), pp. 1-5

A. M. Mathai, R. Naik, M. R. Pai, S. Rai, P. Baliga (2008),
“Microwave histoprocessing versus conventional histoprocessing”,
Indian Journal of Pathology and Microbiology, 51-1, 12-16.

Deborah Duckworth (2009),
“Microwave Processing in the Histology Lab”,
Pathology at John Hopkins.

The Royal Liverpool Hospital (2008),
“Pathos processing: the Liverpool experience”,
Pathology in Practise.

E. Parkin, S. Hindocha, D. Subar, I. Mehrban, J. Walls (2010),
"An Initial Experience with Rapid Microwave Processing in the One-Stop Breast Clinic",
Socie ́te ́ Internationale de Chirurgie, DOI 10.1007/s00268-010-0747-2.

Basavaradhya Sahukar Shruthi, Palani Vinodhkumar, Bina Kashyap, Padala Sridhar Reddy (2013),
"Use of microwave in diagnostic pathology",
Journal of Cancer Research and Therapeutics - July-September 2013 - Volume 9 - Issue 3

Amanda Bonda, John C. Kinnamona (2013),
"Microwave processing of gustatory tissues for immunohistochemistry",
Journal of Neuroscience Methods 215 (2013) 132– 138

Joris P. Bulte, Lenny Polman, Margrethe Schlooz-Vries, Annelies Werner, Renate Besselink, Kelly Sessink, Roel Mus, Susanne Lardenoije, Mechli Imhof-Tas, Johan Bulten, Adriana C. H. van Engen-van Grunsven, Ewout Schaafsma, Luc J. A. Strobbe, Peter Bult, Johannes H. W. De Wilt (2012),
"One-day core needle biopsy in a breast clinic: 4 years experience",
Breast Cancer Res Treat DOI 10.1007/s10549-012-2372-2

Lynne Doverty and Linda Davidson (2013),
"Evaluation of Xylene-free Tissue Processing on the LOGOS all-in-one Hybrid Tissue Processor",
Pathology Department Aberdeen Royal Infirmary, Poster

Linda Graham (2012),
"Microwave Processing for Electron Microscopy",
Anatomical Pathology, LabPlus.

Poster Richard J Zarbo, Ruan C Varney, Michael J Dib, Beverly Mahar and Jason Wozniak (2012),
"Validation of Histology Tissue Processing and Stain Quality of Logos Rapid−Cycle Microwave Processor in Lean Continuous Flow Operations",
Department of Pathology, Henry Ford Health System, Detroit, Michigan, Poster

RJ Zarbo, NS Gupta, R Varney, N Main, S Richard, B Mahar, ON Kryvenko, S McMahon, N Draga, O Alassi, A Ormsby, R D'Angelo (2011),
"Optimization of fixation and processing of biopsy gun prostate needle biopsy specimens",
Department of Pathology, Henry Ford Health System, Detroit, Michigan, Poster

Ciara Barrett, Francesca Brett, David Grehan, and Micheal B. McDermott (2004),
“Heat-Accelerated Fixation and Rapid Dissection of the Pediatric Brain at Autopsy: A Pragmatic Approach to the Difficulties of Organ Retention”,
Pediatric and Development Pathology 7, 595-600.

Ana Maria Lopez, et al (2009),
“Virtual slide telepathology enable an innovative telehealth rapid breast care clinic”,
Human Pathology, 40, 1082-1091, 2009.

Richard Zarbo, et al. (2011),
“Integration of microwave technology to redure fixation and processing time of robotic prostatectomy specimens for whole mount examination”,
USCAP poster, 2011.

Elzabeta Slodkowska et al. (2016),
“Breast Biomarkers can be reliably tested on tissue fixed and processed by Same Day automated Microwave-assisted method”,
PUSCAP poster, 2016.

Salinas et al. (2014),
“Comparacion entre el histoprocesamiento convencional y en Microondas KOS por medio de tincion de rutina, Histoquimica e Inmunohistoquimica en Maxilare de ratones Mus Musculus”,
Trial from the University of Chile, Pathology department, 2014.

A.S Leong, et al. (2006),
“Refinement of Immunological parametes for HER2/neu scoring validation by FISH and CISH”,
Appl Immunohistochem Mol Morphol, 2006.

Linda Graham, LabPLUS (2011),
“Microwave processing for Electron Microscopy”,
Trial poster, 2011.

Antigen Retrieval IHC/ISH

A. S-Y Leong, E. Lee, Z. Haffajee (2002),
“Superheating Antigen Retrieval”,
Applied Immunohistochemistry & Molecular Morphology, 10 - 3 - pp. 263-268

A. S-Y Leong, Z. Haffajee, M. Clarke (2007),
“Microwave Enhancement of CISH for HER2 Oncogene”,
Applied Immunohistochemistry & Molecular Morphology, 15 -1 - pp. 88-93

Electron Microscopy Process of Specimens

J. A. Shröder (2008),
“Clinical Electron Microscopy”,
G.I.T. Imaging & Microscopy, 2/2008, pp 32–35.

J.A. Schroeder, H.R. Gelderblom, B. Hauroeder, Ch. Schmetz, J. Milios and F. Hofstaedter (2004),
“Same-Day EM-Diagnosis of Tissues and Potential Bioterrorism Samples by the Use of Microwave-Assisted Processing”,
Microsc Microanal, Microscopy Society of America, 10 (Suppl 2).

J. A. Schroeder, H. R. Gelderblom, B. Hauroeder, C. Schmetz, J. Milios, F. Hofstaedter (2005),
“Microwave-assisted tissue processing for same-day EM-diagnosis of potential bioterrorism and clinical samples”,
Elsevier Ltd., Micron 37, pp. 577-590.

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